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KMID : 1007520150240031069
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Food Science and Biotechnology
2015 Volume.24 No. 3 p.1069 ~ p.1075
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Protective Effect of Butanol Extracts of Skin of Anguilla japonica against Endoplasmic Reticulum Stress-induced Insulin Resistance via the AMPK Pathway in L6 Myotubes
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Na Min-Kyun
Jeong Yong-Tae
Li Xian
Jin Fansi
Hwang Seung-Lark
Kim Geum-Jin
Yang Ju-Hye
Chang Young-Chae
Kim Dong-Soo
Kim Cheorl-Ho
Chang Hyeun-Wook
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Abstract
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The effect of butanol extracts of the skin of Anguilla japonica (BESA) on endoplasmic reticulum (ER) stress-induced insulin resistance in L6 myotubes was investigated. Western blotting revealed that BESA increased phosphorylation of AMP-activated protein kinase (AMPK) and acetyl-CoA carboxylase, and stimulated glucose uptake in L6 myotubes. Stimulation of AMPK and glucose uptake by BESA were significantly (p<0.05) reduced by siRNA LKB1 or siRNA AMPK, compared with controls, suggesting that enhanced glucose uptake by BESA was predominantly accomplished via an LKB1-mediated AMPK activation pathway. In addition, BESA effectively reduced phosphorylation of ER stress markers, RNA-activated protein kinaselike ER resident kinase, JNK, and significantly (p<0.01) increased glucose uptake via AMPK activation in tunicamycin-treated L6 myotubes, compared with controls. Improvement of insulin sensitivity under ER stress conditions by BESA is predominantly accomplished via an LKB1- AMPK-dependent pathway.
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KEYWORD
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AMP-activated protein kinase, Anguilla japonica skin, Insulin resistance, L6 myotube
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